Dynamiker Biotechnology(Tianjin) Co., Ltd.

Rapid immunochromatographic test for the detection of capsular polysaccharide antigens of Cryptococcus species complex (Cryptococcus neoformans and Cryptococcus gattii) in human serum and cerebral spinal fluid (CSF).

The product uses lateral flow technique and double antibody sandwich format. Cryptococcus antibodies conjugated with gold particles are coated on fiberglass. Test line (T) and control line (C) on nitrocellulose membrane are coated with Cryptococcus antibodies and goat anti-mouse antibodies, respectively. Cryptococcal antigens in samples form antigen-antibody complexes with gold-conjugated antibodies and migrate further along on nitrocellulose membrane. Cryptococcus antibodies immobilized on Test line (T) interact with the above complexes and causing a visible red line. The wicking of samples cause free gold-conjugated antibodies to reach control line (C) and form red color. The absence of cryptococcal antigens in negative samples only cause color change in control line (C). Therefore, control line is included as the quality control standard to ensure the test is visually validated to be functioning correctly.